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GERANYL DIPHOSPHATE SYNTHASE LARGE SUBUNIT, AND METHODS OF USE

机译:磷酸二氢芳基酯合酶大亚基及其使用方法

摘要

A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl disphosphate synthase large subunit. In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthaxe large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.
机译:已经分离并测序了编码来自薄荷的香叶基二磷酸合酶大亚基的cDNA,并确定了相应的氨基酸序列。提供了可复制的重组克隆载体,其编码香叶基二磷酸合酶大亚基。在另一方面,提供了经修饰的宿主细胞,其已用重组克隆载体和/或编码包含分离的重组香叶基二磷酸香叶素大亚基的香叶基二磷酸合酶蛋白的DNA克隆和/或DNA序列进行了转化,转染,感染和/或注射。在另一方面,本发明提供了分离的重组香叶基二磷酸合酶大亚基蛋白和分离的重组香叶基二磷酸合酶小亚基蛋白。因此,提供了用于重组表达香叶基二磷酸合酶的系统和方法。

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