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RAPID GROWING MICROORGANISMS FOR BIOTECHNOLOGY APPLICATIONS

机译:快速增长的生物技术应用中的微生物

摘要

The present invention provides novel rapid growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The rapid growing microorganisms of the invention preferably do not contain detectable levels of bacteriophage genetic material from at least one bacteriophage or in the alternative are resistant to infection by one or more bacteriophage types. The invention also relates to kits and compositions used in the methods of the invention.
机译:本发明提供了新颖的快速生长的微生物及其用于克隆或亚克隆核酸分子的方法。本发明的快速生长的微生物比分子生物学中通常使用的微生物更快地形成菌落,因此在分子生物学中广泛使用的体外克隆方法中提供了显着的改善。本发明的快速生长的微生物优选地不包含来自至少一种噬菌体的可检测水平的噬菌体遗传物质,或者可替代地,其抵抗一种或多种噬菌体类型的感染。本发明还涉及用于本发明方法的试剂盒和组合物。

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