METHODS FOR THE PREPARATION OF POLYNUCLEOTIDE LIBRAIRIES AND IDENTIFICATION OF LIBRARY MEMBERS HAVING DESIRED CHARACTERISTICS

摘要

Methods of directed fragmentation of polynucleotides combined with fragment interchange and ligation are provided for the preparation of polynucleotide libraries as shown in figure 1. Fragmentation can be facilitated by at least one oligonucleotide adapter capable of directing polynucleotide cleavage at homologous sites among a set of parent polynucleotides. Libraries generated by the above methods can be screened for polynucleotides with desired characteristics or properties.