A process for the extra-cellular secretory production of Zymomonas mobilis levansucrase using a transformed Hansenular polymorpha

摘要

PURPOSE: A process for extracellular secretory production of Zymomonas mobilis derived levansucrase using transformed Hansenula polymorpha is provided, thereby producing Zymomonas mobilis derived levansucrase by fed-batch fermentation of transformed Hansenula polymorpha in higher yield. CONSTITUTION: A process for extracellular secretory production of Zymomonas mobilis derived levansucrase using transformed Hansenula polymorpha comprises the steps of: connecting Zymomonas mobilis derived levansucrase gene(levU) with inulase secretion signal sequence, inserting the nucleotide sequence into a vector pDLMOX to prepare an expression vector pDLMOX-Lev; transforming Hansenula polymorpha with the expression vector pDLMOX-Lev to produce Hansenula polymorpha A16/pDLMOX-lev(KCTC 10284BP); and fed-batch fermenting Hansenula polymorpha A16/pDLMOX-lev(KCTC 10284BP) using methanol.