PROTEIN CHIP FOR ANALYZING INTERACTION BETWEEN PROTEIN AND SUBSTRATE PEPTIDE THEREFOR

摘要

PURPOSE: A protein chip for analyzing interaction between protein and substrate peptide therefor is provided, thereby rapidly and enormously analyzing interaction between protein and substrate peptide therefor by improving interaction between small molecular weight of peptides and large molecular weight of enzyme proteins, and between peptides and reactive antibodies. CONSTITUTION: The protein chip for analyzing interaction between protein and substrate peptide therefor is prepared by fixing a substrate peptide on a substrate such as aldehyde slide via a linker protein such as leptin or malic enzyme, wherein the substrate peptide is fused with the linker protein in a type of peptide monomer, dimmer of monomer-proline-monomer, or proline-linked polymer; and the peptide monomer is Camtide having the amino acid sequence set forth in SEQ ID NO: 1 or Ab1 having the amino acid sequence set forth in SEQ ID NO: 8. The method for analyzing interaction between protein and substrate peptide therefor comprises the steps of: adding a protein such as enzyme or antibody which specifically binds to the substrate peptide fixed on a protein chip into the protein chip; and detecting the interaction between the protein and substrate peptide therefor by using a fluorescence-labeled enzyme.