METHOD FOR ISOLATION AND PURIFICATION OF SEROTONINS, LIGNANS AND FLAVONOIDS FROM SAFFLOWER(CARTHAMUS TINCTORIUS L.) SEEDS TO PRODUCE SEROTONIN DERIVATIVES AND PHENOLS MASSIVELY WITH HIGH WORK EFFICIENCY

摘要

PURPOSE: A method for isolation and purification of serotonins, lignans and flavonoids from safflower(Carthamus tinctorius L.) seeds is provided, thereby mass producing serotonin derivatives and phenols from safflower seeds with high work efficiency. CONSTITUTION: The method for isolation and purification of serotonins, lignans and flavonoids from safflower(Carthamus tinctorius L.) seeds comprises the steps of: roasting dried safflower seeds, and removing fat from the roasted safflower seeds by using an oil press or by adding 100% normal-hexane into the safflower seeds and heating the mixture for 2 hours; adding 80% water-soluble ethanol into the fat-removed safflower seeds, heating the mixture at 70 to 90 deg. C, and concentrating the extract under reduced pressure; treating the concentrate with hot water, and adding pectinase into the hot water treated concentrate to hydrolyze it; and passing the hydrolyzed product through the Diaion HP-20 resin, eluting the resin with 60% to 100% water-soluble ethanol, and subjecting the eluate to Sephadex LH-20 column chromatography.