A METHOD FOR CULTURING PORCINE EPIDEMIC DIARRHEA VIRUS AND LIVE VACCINE THEREOF BY USING PORCINE AMINOPEPTIDASE N

摘要

PURPOSE: Provided are a method for culturing porcine epidemic diarrhea virus(PEDV) using porcine aminopeptidase N and an attenuated vaccine thereof, thereby the PEDV can be cultured in higher concentration and the attenuated vaccine having improved immunogenecity can be produced. CONSTITUTION: The method for culturing porcine epidemic diarrhea virus(PEDV) using porcine aminopeptidase N comprises the steps of: culturing animal cells used for proliferation of PEDV; and inoculating PEDV into the cultured animal cell in a medium containing a cell receptor of PEDV and culturing it, wherein the animal cell is a vero cell; the cell receptor of PEDV is a receptor of porcine enterocyte or porcine aminopeptidase N; the medium for proliferation of PEDV contains -MEM, 3% tryptose, phosphate broth, 0.3% yeast extract and antibiotics-antimycotic mixture, and has pH 7.2; and the attenuated vaccine for PEDV contains PEDV and the cell receptor of PEDV.