Plant regeneration method for mature seed-derived callus in zoysiagrass

摘要

A fully differentiated plant is regenerated from undifferentiated plant tissue such as callus obtained from mature seeds, immature embryos, stems, roots or leaves. The invention mature seed-reproducing method provides a plant derived from callus. Reproducing method contains a 2 mg / L of 2,4-dichlorophenoxyacetic acid, 0.2 mg / L of 6-benzyl-amino-purine, 4 mg / L thiamine -HCl and 100 mg / L of α- ketoglutaric acid of and in a MS medium supplemented with the induction of callus. Plant regeneration efficiency was highest when the callus is to be grown in the MS medium supplemented with 3% of maltose and 1 mg / L 6- benzylamino purine or 1 T dia juron (TDZ) in mg / L of. The present invention may be used to introduce genes with important agronomic value as Korean lawn or possibly closely related species Turf Grass.