首页> 外国专利> METHOD OF MANUFACTURING KIT FOR ISOLATING NUCLEIC ACIDS OR BIOLOGICAL MATERIALS, KIT MANUFACTURED BY THE METHOD, AND APPARATUS USING THE KIT

METHOD OF MANUFACTURING KIT FOR ISOLATING NUCLEIC ACIDS OR BIOLOGICAL MATERIALS, KIT MANUFACTURED BY THE METHOD, AND APPARATUS USING THE KIT

机译:制造用于分离核酸或生物材料的套件的方法,由该方法制造的套件以及使用该套件的装置

摘要

The present invention relates to a device for use with the method for producing a kit for separating and purifying a nucleic acid or a variety of biological materials, and the kit prepared by the method, and kit.; Using a solid material having the property of binding to a nucleic acid method for preparing a kit for separation or purification of a nucleic acid or a biological material from a biological sample, (a) the solid material suitable for separation of nucleic acid or a biological material with a buffer the, the steps for producing a container having a plurality of chambers adapted to hold (b) a bore is through-formed and magnetic for collecting said solid material has a projection that is dipped into each of the chambers of the container, and through the bore phase and, (c) step and, (d) filling the chamber with the solid material and buffer to separate nucleic acids and a biological material according to a predetermined procedure given that the bar is manufactured the cover is configured to enter into the chamber a step of sealing the container with a sealing material, (e) includes the step of wrapping the container and the lid in a separate case And that is characterized.; According to the invention, it is simple, of low cost, and, to prepare a small quantity of sample treatment an effective kit. Therefore, clinical or pipette (pipette) of the manual work done by those not sufficiently trained in biological experiments is able to be avoided. In addition, the disposable kit having a column that is pre-filled with an isolated nucleic acid from biological samples can be directly to a clutch between the common PCR to carry out the PCR amplification also without changing the buffer or other tube.
机译:本发明涉及一种与用于生产用于分离和纯化核酸或多种生物材料的试剂盒的方法一起使用的装置,以及通过该方法和试剂盒制备的试剂盒。使用具有与核酸结合的性质的固体材料的方法,用于制备用于从生物样品中分离或纯化核酸或生物材料的试剂盒,(a)适于分离核酸或生物材料的固体材料带有缓冲剂的材料,用于制造具有多个适于容纳(b)孔的腔室的容器的步骤是通孔成型的,并且用于收集所述固体材料的磁性具有浸入到容器的每个腔室中的凸起,并且,通过钻孔阶段,(c)步骤,以及(d)根据给定杆的制造步骤,按照预定程序,用固体材料和缓冲液填充腔室,以分离核酸和生物材料,盖被配置为进入腔室的步骤是用密封材料密封容器的步骤,(e)包括将容器和盖包裹在单独的壳体中的步骤。根据本发明,它是简单的,低成本的,并且是制备少量样品处理的有效试剂盒。因此,能够避免由在生物学实验中未受过足够训练的人员完成的临床工作或移液器(移液器)。另外,具有预先填充有来自生物样品的分离的核酸的柱的一次性试剂盒可以直接连接至普通PCR之间的离合器以进行PCR扩增,而无需改变缓冲液或其他试管。

著录项

  • 公开/公告号KR100445560B1

    专利类型

  • 公开/公告日2004-08-21

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20010067742

  • 发明设计人 장기영;

    申请日2001-10-31

  • 分类号C12Q1/68;

  • 国家 KR

  • 入库时间 2022-08-21 22:46:45

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