Processes for efficient regeneration of zyosiagrass plants by tissue culture

摘要

PURPOSE: Provided is a process for efficient redifferentiation of Zoysia japonica Steud. by tissue culture, thereby increasing regeneration rat and providing Zoysia japonica Steud. without somatic cell mutation. CONSTITUTION: The process for efficient redifferentiation of Zoysia japonica Steud. is characterized by comprising the steps of: inducing callus from a seed of Zoysia japonica Steud. in an MS medium(pH 5.8) containing 2,4-D 1-4mg/L. BAP 0-4mg/L, thiamine-HCl 4mg/L and alpha-ketogultaric acid 100mg/L; growing the callus in an MS medium(pH 5.8) containing 2,4-D 0.5mg/L. BAP 0-4mg/L, thiamine-HCl 4mg/L and alpha-ketogultaric acid 100mg/L; and redifferentiating a plant therefrom in an MS medium(pH 5.5-6.2, preferably 5.8) containing maltose 15-45g/L or glucose 15-45g/L, BAP 1-3mg/L or TDZ 1-3mg/L.