Detection of Microcystin Producing-Cyanobacteria by PCR

摘要

PURPOSE: A detection method of cyanobacteria producing microcystin by using PCR is provided, thereby detecting a small amount of microcystin-producing Cyanobacteria. Therefore, water quality can be appropriately maintained. CONSTITUTION: A primer capable of amplifying a specific region of a peptide synthetase gene that is specifically present in the microcystin producing Cyanobacteria is provided, wherein the specific region of the peptide synthetase gene is adenylation domain; the primer is a pair of primers consisting of one primer selected from MCF-1 having the nucleotide sequence of SEQ ID NO: 1 or MCF-2 having the nucleotide sequence of SEQ ID NO: 2, and another primer selected from MCR-1 having the nucleotide sequence of SEQ ID NO: 3 or MCR-2 having the nucleotide sequence of SEQ ID NO: 4. A detection method of microcystin-producing Cyanobacteria by using PCR comprises the steps of: (1) extracting DNAs from various microorganisms; (2) PCR amplifying the DNAs as template using a primer capable of amplifying a specific region of the peptide synthetase gene; (3) subjecting the amplified genes to electrophoresis; and (4) dyeing of the developed gene to analyze the PCR product.