METHOD FOR INTEGRATION OF MULTIPLE POLYMERASE REACTIONS OF AMPLIFICATION WITH FOLLOWING ANALYSIS OF AMPLIFIED SEQUENCES ON BIOCHIP DIRECTLY

摘要

FIELD: molecular biology, biochemistry. SUBSTANCE: invention proposes the new method for analysis of any nucleotide sequences with oligonucleotides immobilized into separate gel cells. This method allows coinciding the amplification process of sequences to be tested with analysis of amplified products inside of each members of chip. For this purpose specific sets of primers are immobilized in different cells that, except for standard constantly immobilized primers comprise definite amount of modulated primers (they can be released, activated, inactivated) and that can be used by necessary for both multiplication and for analysis of cell-specific DNA fragment. Enzymatic reactions are carried out parallel into separate gel members covered and isolated each from other by mineral oil. New method provides possibility for simultaneous analysis of multiple of different nucleotide sequences. Invention can be used in scientific-research and medicinal practice. EFFECT: valuable method of analysis. 34 cl, 15 dwg, 1 tbl