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METHOD FOR ASSAY OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C3 BY ALTERNATIVE PATHWAY OF ACTIVATION
METHOD FOR ASSAY OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C3 BY ALTERNATIVE PATHWAY OF ACTIVATION
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机译:通过替代性激活途径评估人类补体成分C3的功能活性的方法
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摘要
FIELD: medicinal immunology.;SUBSTANCE: invention relates to methods for assay of functional activity of complement factors in human serum blood and can be used in diagnosis of some diseases and in biological preparations. Method involves sorption of activator of the complement alternative pathway in microplate wells and then solution containing reagent R3 and analyzed sample containing component C3 of human complement with unknown activity are added. Incubation is carried out in the presence of Mg2+ ions and in the absence of Ca2+ and then conjugate of enzyme with antibodies raised against the human component C3 and substrate for the same enzyme are added. The calculation of C3 activity is carried out by the amount of formed product of enzymatic reaction. Invention provides the development of method allowing the assay of functional activity of component C3 in human complement system in alternative pathway of activation.;EFFECT: improved assay method.;1 tbl, 5 ex
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机译:技术领域本发明涉及测定人血清中补体因子功能活性的方法,可用于某些疾病的诊断和生物制剂中。该方法涉及在微孔板孔中吸附补体替代途径的激活剂,然后添加含有试剂R3的溶液和含有未知活性的人补体组分C3的分析样品。在Mg 2 + Sup>离子存在且Ca 2 + Sup>不存在的条件下进行孵育,然后将酶与针对人C3的抗体和底物结合添加相同的酶。 C 3活性的计算是通过酶促反应形成产物的量来进行的。本发明提供了方法的发展,该方法允许在替代激活途径中测定人补体系统中组分C3的功能活性。效果:改进的测定方法。1 tbl,5 ex
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