Manufacturing sample carrier with points for matrix-assisted laser desorption and ionization, forms MALDI matrix points by gas phase sublimation

摘要

The MALDI matrix points are formed by depositing a MALDI matrix substance from the gas phase by sublimation, onto the sample carrier (1). A mask is used during sublimation, to form the points. It includes openings depositing corresponding data. This comprises e.g. the composition of the matrix substance and/or provides alignment points. The matrix points are aligned along a raster; they may have substructures and be distributed in isolated, divided points. Single- or diverse matrix substances are deposited. The sample carrier has an ultraphobic surface. Points and divided points upon it, are hydrophilic, and are completely surrounded by ultraphobic regions. The deposit is multi-layered, comprising a first layer (2) with ultraphobic surface (3) and a substrate layer (4). The first layer is applied reversibly to the substrate. Departure from planarity is less than 100 microns over a length of 100 mm. The first layer is adhered-on (5). There is an electrical contact between the first layer and the substrate. Independent claims are included for the planar structure so obtained, a corresponding structure with long term stability, in an impermeable, opaque, evacuated enclosure, and a structure with additional biological material on the matrix points.