Protein-encapsulated, insoluble, gas-containing microspheres, their production and use as imaging agents for ultrasound

摘要

Encapsulated gas microspheres having improved pressure resistance and stability are made by: mixing an aqueous solution of a filmogenic protein such as human serum albumin with a water insoluble gas such as perfluoropropane and subjecting the mixture to ultrasonic or mechanical cavitation in the absence of oxygen in an apparatus that is closed to the atmosphere.