IMMUNONZYMATIC ASSAY FOR DIAGNOSIS OF PFERDEAN MIE-VIREN CANCELLATION WITH THE HELP OF RECOMINANT VIRUSPROTEIN RGP90

摘要

The present invention relates to a method and kit of detecting antibodies in the serum of animals infected with equine infectious anemia virus using the immunodiagnosis with the recombinant viral antigen gp90. The antigen was bound to solid supports (microtitter plates, tubes, beads or nitrocelullose papers or nylon) and reacted with clinical samples. After incubation with conjugated (Anti-equine immunoglobulin-enzyme) the reaction was revealed with a solution composed of the substract of the enzyme used in the conjugate (cromogene). After development of the reaction (color formation) it was stopped with acid solution and measured. The immunoassay may be a direct second antibody immunoassay, and one or two step sandwich immunoassay.