Multiplex detection and identification of bacteria in complex mixtures or water, useful e.g. for testing food, by simultaneous, but spatially resolved amplification

摘要

Process for detecting and identifying bacteria present in a complex mixture or in water by simultaneous but spatially separated, multiplex amplification of target nucleic acids (I). Process for detecting and identifying bacteria present in a complex mixture or in water by multiplex amplification, simultaneous but spatially separated, of target nucleic acids (I) comprises: (1) contacting the sample of (I) with at least 2 pairs of primers for amplification, each pair being specific and exclusive for (I) of a bacterial species, genus or group; (2) amplification of (I); and (3) detecting and identifying bacteria in the mixture. Independent claims are also included for the following: (1) similar process that includes quantification of the bacteria, where amplification is by real-time PCR; (2) eleven primer pairs (sequences defined in the specification) for the process; (3) eleven sets of primer pairs and a probe (sequences defined in the specification) for the process; (4) probes for the process, specific and exclusive for a bacterial species, genus or group, that are complementary to amplification products generated by primers of (2); (5) kit for determining, identifying and optionally quantifying bacterial nucleic acid that contains at least 2 each of the new primer pairs and new probes; and (6) nucleic acid microarray or chip for identifying bacteria by the new process.