Preparing composition for delivering macromolecules to cells, useful e.g. in gene therapy, comprises adsorbing a transducer peptide on to hydrophobic domains on the cargo

摘要

Preparing a composition (A) for introducing, into a living cell, a cargo (B) comprising a macromolecule or a molecular assembly of largest dimension not over 1 micron and having one or more hydrophobic domains (HD) on its surface, is new. Preparing a composition (A) for introducing, into a living cell, a cargo (B) consisting of a macromolecule or a molecular assembly of largest dimension not over 1 micron and having one or more hydrophobic domains (HD) on its surface. The method comprises adsorption, on HD, of at least one transducer peptide (I), excluding those of 16-30 amino acids (aa) that contain an HD of 3-5 Trp residues (including at least one Trp-Trp pair), alternating with Glu or Thr residues, and a hydrophilic domain of 4 or 5 consecutive basic residues. An Independent claim is also included for (A) prepared by the new process.