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Medium for detecting and identifying microorganism, useful particularly for Salmonella in diagnosis or food testing, includes an inhibitor that inactivates enzymes that may cause false positives

机译:用于检测和鉴定微生物的介质,特别是在诊断或食品检测中对沙门氏菌有用的介质,包括一种抑制剂,该抑制剂可使可能导致假阳性的酶失活

摘要

Medium (A) for detection and/or identification of microorganisms (B) comprises: (a) culture medium; (b) at least one substrate (S) that is hydrolyzed to a labeled product (LP) by an enzyme (E1) specific for (B); and (c) the new feature, at least one inhibitor (I) of at least one enzyme (E2) that is different from E1 or the same as E1 but not specific for (B). An independent claim is also included for a process for detection/identification of (B) by inoculating (A) with test organism, incubation and detecting (B) from hydrolysis of at least one substrate to LP.
机译:用于检测和/或鉴定微生物(B)的培养基(A)包括:(a)培养基; (b)至少一种底物(S),其被(B)特异性的酶(E1)水解成标记产物(LP); (c)新特征,至少一种酶(E2)的至少一种抑制剂(I),该酶不同于E1或与E1相同,但对(B)没有特异性。还包括通过以下方式进行检测/鉴定(B)的方法的独立权利要求:通过用待测生物接种(A),温育和检测(B)至少一种底物水解成LP。

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