VARIANT FORM OF HEAT-STABLE HYGROMYCIN PHOSPHOTRANSFERASE

摘要

PROBLEM TO BE SOLVED: To provide a heat-stable and drug-resistant gene easily usable as a selection marker in an extremely thermophilic bacterium, especially in Thermus bacterium, and especially imparting resistance against drugs except kanamycin.;SOLUTION: The gene encoding a heat-stable variant form of a hygromycin phosphotransferase exhibiting high heat stability compared to that of hygromycin B is obtained by causing variation of a hygromycin phosphotransferase gene of Escherichia coli.;COPYRIGHT: (C)2005,JPO&NCIPI