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DNA ARRAY, METHOD FOR ANALYZING GENE EXPRESSION USING THE SAME AND METHOD FOR MAKING SEARCH OF USEFUL GENE

机译:DNA阵列,利用其分析基因表达的方法和寻找有用基因的方法

摘要

PPROBLEM TO BE SOLVED: To provide a method for comprehensive analysis of gene expression of an organism having scarce information about the base sequence as the object with an inexpensive and efficient DNA array and to provide a method for making a search of a useful gene. PSOLUTION: The comprehensive analysis of the gene expression can be carried out without being subjected to restrictions on the amount of information about genetic sequence at all by directly immobilizing a genomic library prepared from a randomly cleaved genomic DNA fragment on a substrate providing individual identification and correspondence. Furthermore, a sub-DNA array in which a plasmid detected with the prepared random genomic DNA array is fragmented into a shorter DNA and immobilized on the substrate is prepared and the gene expression is analyzed to make the search of the useful gene. PCOPYRIGHT: (C)2005,JPO&NCIPI
机译:<要解决的问题:提供一种方法,以廉价且有效的DNA阵列全面分析具有关于碱基序列的信息少的生物体的基因表达,并提供一种用于寻找有用的方法的方法。基因。

解决方案:通过直接将由随机切割的基因组DNA片段制备的基因组文库直接固定在提供个体的底物上,可以对基因表达进行全面分析而完全不受基因序列信息量的限制。识别和对应。此外,制备亚DNA阵列,其中用制备的随机基因组DNA阵列检测到的质粒被片段化成较短的DNA并固定在底物上,并且分析基因表达以寻找有用的基因。

版权:(C)2005,JPO&NCIPI

著录项

  • 公开/公告号JP2005224103A

    专利类型

  • 公开/公告日2005-08-25

    原文格式PDF

  • 申请/专利权人 HITACHI LTD;

    申请/专利号JP20040032813

  • 申请日2004-02-10

  • 分类号C12N15/00;C12M1/00;C12M1/34;C12N15/09;C12Q1/68;G01N33/53;G01N37/00;

  • 国家 JP

  • 入库时间 2022-08-21 22:35:37

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