IMANITIB-RESISTANT PHILADELPHIA CHROMOSOME POSITIVE ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE AND USE THEREOF

摘要

PROBLEM TO BE SOLVED: To establish a stable human cell line exhibiting resistance to imanitib mesylate at a high concentration and to provide a method for screening a therapeutic agent for imanitib mesylate-resistant human leukemia by using the human cell line.;SOLUTION: The Ph (Philadelphia chromosome) positive human acute lymphoblastic leukemia cell line characterized as resistant to the imanitib mesylate at a concentration of at least 5 μM is provided. In a preferred embodiment, the chromosome is characterized as follows. The chromosome contains a BCR/ABL gene and has a point mutation of an ATP binding site of a gene product. In the point mutation, threonine at the 315th residue in an amino acid sequence of sequence number 2 is substituted by isoleucine. In an especially preferred embodiment, a cell line TCC-Y/sr is provided.;COPYRIGHT: (C)2005,JPO&NCIPI