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METHOD FOR SUBCULTURE OF GINSENG CALLUS

机译:人参愈伤组织的代偿方法

摘要

PROBLEM TO BE SOLVED: To provide a method for the subculture of ginseng callus, enabling the change in the properties of seed stocks to be suppressed to a minimum even if a long-term subculture is performed, enabling the activity of seed stocks to be maintained at high levels, as a result, capable of suppressing such problems as to decrease in the harvest of a ginseng callus-cultured product after subculture and/or decrease in the content of secondary metabolites such as saponins produced inside of the ginseng callus-cultured product.;SOLUTION: The method for the subculture of ginseng callus comprises alternately repeating a liquid culture under conditions A and that under conditions B, wherein the conditions B are such that, as compared to the conditions A, the amount of a medium to be used is 110-200 vol.% of that in the conditions A and the concentration of an auxin in the liquid medium is 1/7 to 1/2 time that in the conditions A.;COPYRIGHT: (C)2006,JPO&NCIPI
机译:解决的问题:提供一种人参愈伤组织继代培养的方法,即使进行长期继代培养,也能将种苗特性的变化抑制到最小程度,从而保持种苗的活性结果,能够抑制高水平的继代培养后人参愈伤组织培养物的收成减少和/或人参愈伤组织培养物内部产生的皂苷等次生代谢物含量降低的问题。解决方案:人参愈伤组织继代培养的方法包括在条件A和条件B下交替重复液体培养,其中条件B与条件A相比要使用的培养基量浓度为条件A的110-200 vol。%,液体培养基中植物生长素的浓度为条件A的1/7至1/2倍;版权所有:(C)2006,JPO&NCIPI

著录项

  • 公开/公告号JP2005312323A

    专利类型

  • 公开/公告日2005-11-10

    原文格式PDF

  • 申请/专利权人 NITTO DENKO CORP;

    申请/专利号JP20040131678

  • 发明设计人 SAHASHI HIROKO;TSUJIKURA YOSHIKO;

    申请日2004-04-27

  • 分类号C12N5/04;

  • 国家 JP

  • 入库时间 2022-08-21 22:33:38

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