METHOD FOR DETERMINING NUCLEIC ACID BASE SEQUENCE BY USING TIME-OF-FLIGHT SECONDARY-ION MASS SPECTROMETRY

摘要

PROBLEM TO BE SOLVED: To provide a method for analyzing oligo DNA immobilized on a glass substrate by using a time-of-flight secondary-ion mass spectrometry (TOF-SIMS) method.;SOLUTION: This method for determining a nucleic acid base sequence comprises at least processes (1), (2), and (3), as follows. (1) A process for preparing a substrate with a nucleic acid immobilized thereon, the nucleic acid being labeled with an element or an element group not included in the base sequence and the basic sequence of the nucleic acids being desired to be analyzed; (2) a process for measuring the nucleic acid immobilized on the substrate by using the time-of-flight secondary-ion mass spectrometry (TOF-SIMS) method; (3) a process for clarifying the base sequence of the nucleic acid based on a molecular weight increment from the molecular weight of a labeled substance by utilizing the fact that fragments of a nucleic acid sequence including the labeled substance includes a nucleic acid sequence different in chain length.;COPYRIGHT: (C)2005,JPO&NCIPI