TRANSLATION REACTION-PROMOTING DNA FRAGMENT AND TEMPLATE DNA USING THE SAME, mRNA, AND REACTION LIQUID FOR CELL-FREE-BASED PROTEIN SYNTHESIS

摘要

PROBLEM TO BE SOLVED: To provide a DNA fragment capable of improving translation efficiency without depending on a cap structure in synthesizing a cell-free-based protein using an extract liquid for the purpose containing an arthropod-derived extract such as a silkworm extract liquid or insect cultured cell extract liquid.;SOLUTION: The DNA fragment having a specific base sequence is to be used for translation reaction promotion in the cell-free-based protein synthesis. Another version of the DNA fragment equivalent to the above fragment and having translation reaction-promoting activity is also provided. A template DNA using the DNA fragment is also provided. A mRNA is also provided. A reaction liquid for the cell-free-based protein synthesis is also provided.;COPYRIGHT: (C)2005,JPO&NCIPI