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METHOD FOR DETERMINING WEIGHT AVERAGED MOLAR MASS, MEAN SQUARE RADIUS AND SECOND VIRIAL COEFFICIENT OF UNFRACTIONATED SAMPLE
METHOD FOR DETERMINING WEIGHT AVERAGED MOLAR MASS, MEAN SQUARE RADIUS AND SECOND VIRIAL COEFFICIENT OF UNFRACTIONATED SAMPLE
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机译:确定未破碎样品平均重量的摩尔质量,均方半径和第二虚系数的方法
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摘要
PROBLEM TO BE SOLVED: To provide a new method for measuring molecular properties of an unfractionated solution of macromolecules, which shares some similarities with a standard Zimm plot technique.;SOLUTION: The method begins with a preparation of several sample aliquots spanning a range of concentrations. The aliquots are then injected sequentially into a stream such as provided by a liquid chromatograph. Each aliquot produces, thereby, an effective "peak" where the element corresponds to a different concentration of the diluted aliquot. By analyzing the angular and concentration dependence of the scattered signals throughout the corresponding peaks, the weight averaged molar mass, the mean square radius, and the second virial coefficient can be derived. In contrast to earlier on-line methods, higher precision is achieved, while using a smaller quantity of samples.;COPYRIGHT: (C)2005,JPO&NCIPI
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