The mutant DNA polymerase I mutants, and using a mutated DNA polymerase I mutant in a continuous culture method for random mutagenesis is targeted

摘要

(The polymerase has to increase the error rate during replication, mutation of motif B and / or in motif A of the active domain) DNA polymerase I of the mutant. For expressing a low fidelity polymerase mutants of these expression plasmid constructs and cell lines are provided. Also, in order to produce a library (may be of prokaryotic or eukaryotic it) using a low fidelity DNA polymerase I mutants of these genes was randomly mutagenized how to is provided. In continuous culture for causing repeated conveniently includes that coupling the selection and mutagenesis, random mutagenesis, resulting in (distributed along the array wide) base pair substitution in a diverse range This . The several advantages, minimization of adverse damage to the chromosomal DNA, and adaptation to strain accept complementarity is included, the production of (with altered properties) Enzyme This and identification are substantially promoted that.