PROBLEM TO BE SOLVED: To provide a medium for a plant belonging to the genus Cypripedium macranthum enabling its seedlings to be mass-produced at a time through improving germination rate and also suppressing occurrence of their browning decay during culture after germination to raise their survival rate. SOLUTION: A method for culturing a plant belonging to the genus Cypripedium macranthum comprising the steps of sorting seeds, sterilely sowing the seeds mixed in a culture solution on a medium, culturing the seeds until they germinate, making a subculture of the germinated and organ-differentiated seeds by substituting the medium, exposing the cultured seedlings grown in the subculture step to a low-temperature ambience, and potting the thus exposed seedlings comprising transplanting them in soil for their cultivation, involves using the medium which is prepared by incorporating 1,000 mL of an aqueous solution with 1-3 g of a fertilizer containing nitrogen, phosphorus and potassium, 1-3 g of peptone, 10-30 g of saccharides 0.2-1.0 mL of a plant activator and 16-70 mg of vitamins.
展开▼
机译:要解决的问题:为Macranthum属的植物提供一种培养基,通过提高发芽率并抑制发芽后培养过程中褐变的发生,提高其成活率,从而使其批量生产。 。解决方案:一种用于培养Cy兰属植物的方法,该方法包括以下步骤:分选种子,将混合在培养液中的种子无菌播种到培养基上,培养种子直至发芽,然后对发芽的器官进行继代培养。通过替换培养基来分化种子,将继代培养步骤中培养的幼苗暴露于低温环境中,然后将如此暴露的幼苗盆栽起来,包括将其移植到土壤中进行种植,这涉及使用通过掺入1,000 mL含1-3 g含氮,磷和钾的肥料,1-3 g p,10-30 g糖,0.2-1.0 mL植物活化剂和16-70 mg维生素的水溶液。
展开▼