Virus inoculation method using a feed and fee increase the infection rate of the virus to silkworm

摘要

There is provided a process for producing a feed for elevating a rate of infection with a recombinant virus in a large number of silkworms, and a method of simultaneously inoculating orally a recombinant virus into a large number of silkworms. A process for producing a feed for elevating a rate of infection with a recombinant virus in a large number of silkworms includes mixing a powdery synthetic feed with a distilled water in which Tinopal UNPA-GX is dissolved at a final concentration of 0.2 to 0.4% by weight, grinding the mixture by stirring, putting it into a heat-resistant polyethylene bag, and heating it in an autoclave; and a method of simultaneously inoculating orally a recombinant virus to a large number of silkworms reared on a silkworm basket is characterized by including supplying a feed produced by the above process to a large number of 5th-instar silkworms reared on a silkworm basket, and, at 20 to 24 hours later, placing a net carrying a feed coated with a recombinant virus-containing solution thereon on the silkworm basket, thereby supplying the feed coated with the recombinant virus.