Discovery of the drug from its isolation and the culture of microorganisms from the natural environment

摘要

The method of the present invention, the microorganism "No culture (uncultivatible)" previously is is intended for the new use of a diffusion chamber which can be isolated therein. Of the present invention that rather than attempting to replicate the natural environment of an unknown microorganism, includes colonies exposed to all components of the original environment division microorganisms, obtained at the same time the other hand, the isolated microorganisms by this I will provide a way. The method of the present invention, microorganisms are difficult to grow advantage of the recognition that many do not form colonies visible to the naked eye. Therefore, must be isolated in complex expression microscope as "(microcolonies) colonies fine" These organisms. Furthermore, the method according to the invention, co the presence of concomitant (companion) microorganisms - which made possible the isolation of novel microorganism capable of growing in artificial media only in the manner of cultivation.