Monitoring method of purification and polypeptide production that uses surface-enhanced laser desorption ionization mass spectrometry

摘要

The present invention provides a method of monitoring the production of the target polypeptide by creating a surface enhanced laser desorption ionization mass spectral profile of samples taken at different time points from a given batch or sample taken from the cell culture batches. The present invention also provides a method for monitoring the purification of the target polypeptide from the mixture by creating a surface enhanced laser desorption ionization mass spectral profile of samples taken at various purification steps in further. The invention further relates to a method of identifying a condition available to increase the size of a given purification step.