Relative quantitative mannered null description below of a the degree of methylation of the cytosine base

摘要

The invention describes a process for the relative quantification of the methylation of cytosine bases in dna - samples, wherein the following method steps of: dollars a a) an genomic dna - sample with a reagent chemically, 5 - methylcytosine and cytosine react differently according to the reaction and thus a different base-pairing behavior in the dna duplex; dollars a b) it is related to the dna - sample, wherein a fluorescent dctp - derivative is added; dollars a is c) are hybridized to the amplified dna - sample to one or more immobilized oligomers, wherein the immobilized oligomers, in each case complementary to at least one of the primers are used in the amplification step; and dollars a d) is measured the fluorescence of the hybridized amplificates quantitatively.