Method and the device which draw up the sample of organization for histology inspection and pathological inspection

摘要

In order soaks the material in the tank of the coolant, to freeze the material passing the material substantially at fixed specified speed, low temperature it retains the living thing material which has lived by circulating the coolant. The material before the freezing, without processing soaks directly in the coolant, or chemistry processes. Method of this invention, although formation of ice crystal is evaded inside cell structure, freezes the organism material sufficiently, quickly maintains that anatomy structure concerning the sample, leaves the biochemical activity after the freezing. As for temperature of the coolant, although formation of the artefact in the cell membrane by the occurrence and thermal fluctuation of stress destruction is made smallest it is desirable to be between the -30C from the -20C which is sufficiently warm temperature. As for the cell which is frozen making use of the method of this invention, the cell which is frozen by the other low temperature retention which is used with pathological and organizational technique compared to, considerably the cell and the intercellular damage were little. Because in order the material to glass to convert, to freeze the organism material it is possible this invention, biochemical activity inside the cell does not disappear even after the freezing, various execution forms of this invention may use for the device in order to prepare the organism material for the technique whose low temperature histology and immunity histochemistry are new in the CARE of research and the patient field.