Low temperature active alkaline protease, the preparation of microorganisms and the alkaline protease for the production of this

摘要

PROBLEM TO BE SOLVED: To obtain the subject protease retaining high activity even under low-temperature conditions, nearly not inhibited even by various kinds of surfactans, stable in solutions ranging from acidic to highly alkaline ones, thus useful as an ingredient in detergent compositions, for softening meat or aging cheese, etc. ;SOLUTION: This new protease as a low-temperature-active alkali protease, which can be mass-produced by e.g. Bacillus sp. KSM-SP3659 (FERM P-15136), has the following characteristics: (1) acting at 0-40°C, the optimum temperature being at about 30°C (shifting to 40°C in the presence of Ca²⁺), and retaining 15% of its activity at the optimum temperature even at 0°C (in ice water); (2) acting pH range; 5-12 or higher, optimum pH being 11; (3) molecular weight: about 36500 (SDS-PAGE); and (4) acting on casein, hemoglobin, gelatin, etc.;COPYRIGHT: (C)1997,JPO