Synthetic compounds model unresponsive to non-human animals having immunopotentiating action

摘要

And in which gene function encoding recognizing TLR7 synthetic compounds having immunopotentiating effect of imidazoquinoline compounds is deficient on its chromosome, to provide a synthetic compound non-human animal model unresponsive. Constructs a targeting vector by replacing the plasmid carrying the marker gene and poly (A) signal, a gene fragment of all or part of the gene region containing the transmembrane region and the intracellular region of the TLR7 gene obtained from a mouse gene library , and introduced into embryonic stem cells after linearized the targeting vector to produce chimeric mice microinjected into blastocysts of mice, the targeted embryonic stem cell deficient in TLR7 gene function, wild-type mice and the chimeric mice to prepare a TLR7 knockout mice by making a heterozygous mice are mated to door, to take intercrossing heterozygous mice.