Dna synthesis promoters dna polymerase-associated factors and utilization thereof

摘要

A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R²—CH₂—NR¹_xH_y  (1) wherein R¹ is an alkyl group having 1 to 3 carbon atoms, R² is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R⁴ is methyl, hydrogen or forms a pyrrolidine ring when combined with R¹, R⁵ is —CO₂H or —SO₃H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3. ;The present invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or bind to DNA polymerase.