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Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
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机译:通过选择性扩增缺口翻译DNA文库并从复杂的模板混合物中扩增来进行基因组行走
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摘要
Improved methods and reagents for chromosome walking of nucleic acid are discussed herein. A library of amplifiable nick translation molecules is generated, and a chromosome walk is initiated from a known sequence in the nucleic acid by producing at least one nick translate molecule, sequencing part of the nick translate molecule, and producing a second nick translate molecule by initiating the primer extension from the region of the obtained sequence of the prior nick translate molecule.
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