Method of estimating the genotype of fertility recovery locus to rice bt type male fertility cytoplasm

摘要

The invention relates to a method of detecting Rf-1 gene, or the gene that restores the BT type cytoplasmic male sterility which is employed in hybrid rice breeding. More specifically, the invention provides a method of detecting Rf-1 gene by making use of the fact that a plurality of PCR markers present near the locus of Rf-1 gene are linked to said locus. Specifically, the invention genotypes a plurality of PCR marker loci present in the neighborhood of Rf-1 gene based on the fact that the locus of Rf-1 gene is located between novel PCR markers S12564 Tsp509I and C1361 MwoI on chromosome 10 of rice, whereby estimation of the presence or absence of Rf-1 gene and selection of Rf-1 gene homozygous individuals can be performed in a convenient and accurate way.