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Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
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机译:用于鉴定活细胞中蛋白酶,蛋白酶靶位点和蛋白酶活性调节剂的融合蛋白
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摘要
The invention provides a fusion protein including a reporter polypeptide, a linker polypeptide comprising a protease cleavage site, and a repressor polypeptide. The repressor polypeptide represses the activity of the reporter polypeptide by conferring a specific localization in a cell that reduces activity of the reporter activity until the cleavage site is cleaved. A method is also provided for identifying a protease that recognizes a specific protease cleavage site. The invention further provides a method of identifying a compound that activates a protease.
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