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Methods and compositions for detecting cancers associated with methylation of hMLH1 promoter DNA
Methods and compositions for detecting cancers associated with methylation of hMLH1 promoter DNA
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机译:用于检测与hMLH1启动子DNA甲基化有关的癌症的方法和组合物
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摘要
Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
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