The method for identifying animals infected with M. bovis, tracer for detecting antibodies to M. bovis in the test of polarization of fluorescence, and kit for detecting antibodies to M.Bovis in a sample taken from an animal

摘要

"The method for identifying animals infected with M. bovis, tracer for detecting antibodies to M. bovis in the test of polarization of fluorescence, and kit for detecting antibodies to M.Bovis in a sample taken from an animal ". The present invention provides a test for the detection of animals infected with M. bovis.A tracer comprising a peptide of protein MPB70 of M. bovis, conjugated to a fluorophore, is added to a serum sample from one animal to form a mixture.The polarization of fluorescence of the mixture is then measured and compared with the polarization of fluorescence of a control.The present invention provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis.The tracer comprises a peptide of protein MPB70 of M bovis conjugated to a fluorophore, so that the tracer is able to bind antibodies specific for M.Bovis to produce a detectable change in polarization of fluorescence.