Development factor modified protein matrices for tissue construction

摘要

"PROTEIN MATRIXES MODIFIED BY DEVELOPMENT FACTOR FOR FABRIC CONSTRUCTION". Proteins are incorporated into protein or polysaccharide matrices for use in tissue repair, regeneration and / or remodeling and / or drug release. Proteins may be incorporated so that they are released by matrix degradation, diffusion and / or enzymatic action. As demonstrated by the examples, one method is to bind heparin to the matrix by either covalent or non-covalent methods to form a heparin matrix. Heparin then non-covalently binds heparin binding developmental factors to the protein matrix. Alternatively, a fusion protein may be constructed which contains a cross-linking region, such as a factor XIIIa substrate and natural protein sequence. Incorporation of degradable bonds between matrix and bioactive factors can be particularly useful when long-term drug release is desired, for example, in the case of nerve regeneration, where it is desirable to vary the drug release rate spatially as a function. regeneration, for example, slightly close to the living tissue interface and more slowly away within the injury zone. Additional benefits include the lower total drug dose in the delivery system, and spatial release adjustment that allows a larger percentage of the drug to be released at the time of increased cellular activity.