Process for the isolation of peptu00ecdeos using the endopeptidades EC 3.4.24.15 (EP24.15) and EC 3.4.24.16 (ep24.16) recombinant catalytically inactivated by mutation place directed

摘要

Process for isolation of peptu00ecdeos using the Endopeptidases EC 3.4.24.15 (EP24.15) and EC 3.4.24.16 (ep24.16) recombinant catalytically inactivated by mutation place run. The present invention relates to a process for the isolation and identification of certain peptides from tissue samples using recombinant enzymes of animal or vegetable thimetOligopeptidase (EP24.15) or neurolisina (ep24.16).The method described here comprises the isolation of specific peptides from crude extracts of tissues or cells, animals or plants,And allows subsequent identification of such peptides which, once isolated, can be sequ00b3enciados and used for scientific purposes and industrial.