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Process for selectively producing male or female stereotypes

机译:选择性产生男性或女性刻板印象的方法

摘要

"PROCESS FOR SELECTIVELY PRODUCING STERILE MALE OR FEMALE PLANTS". The present invention relates to a process for producing sterile male or female plants comprising the steps of transforming plant material with a polynucleotide encoding at least one enzyme that reacts with a non-phytotoxic substance to produce a phytotoxic substance, and regenerating the material thus transformed into a plant, wherein said non-phytotoxic substance is applied to the plant until the period of formation of male or female gametes and / or maturation, so that the non-phytotoxic substance causes the production of a phytotoxic substance which avoids selectively the formation of or otherwise renders said gametes non-functional, wherein the enzyme is expressed preferentially in both male and female reproductive structures, characterized in that (i) the non-phytotoxic substance is selected from the group consisting of ester derivatives of non-phosphonate herbicides, which herbicides are directly phytotoxic to non-green tissue, D-alpha amino acids, non-protein D-alpha amino acid peptide derivatives, aryloxyphenoxypropionate senantiomers and S-enantiomers of aryloxyphenoxypropionate ester derivatives and (ii) the enzyme is selected from the group consisting of carboxylesterases, D amino acid oxidases, D-amino acid dehydrogenases, D-amino acid racemases, 2-arylpropionyl-CoA epimerases, alpha-methylac-CoA racemases, thiesterases and acyl-CoA synthetases.
机译:“选择性生产无菌男性或女性植物的过程”。本发明涉及一种生产不育雄性或雌性植物的方法,该方法包括以下步骤:用编码至少一种与非植物毒性物质反应以产生植物毒性物质的酶的多核苷酸转化植物材料,并再生如此转化为一种植物,其中将所述非植物毒性物质施用于植物,直至雄配子或雌配子形成和/或成熟为止,从而使非植物毒性物质引起植物毒性物质的产生,从而避免选择性地避免或否则使所述配子失去功能,其中所述酶优先在雄性和雌性生殖结构中表达,其特征在于(i)所述非植物毒性物质选自非膦酸酯除草剂的酯衍生物,该除草剂对非绿色组织,D-alpha氨基酸,非蛋白质D-alpha氨基酸有直接的植物毒性肽衍生物,芳氧基苯氧基丙酸酯酯衍生物的芳氧基苯氧基丙酸酯的对映异构体和S-对映异构体,以及(ii)所述酶选自羧酸酯酶,D氨基酸氧化酶,D-氨基酸脱氢酶,D-氨基酸消旋体,2-芳基丙酰基-CoA差向异构酶,α-甲酰辅酶A外消旋酶,三酯酶和酰基辅酶A合成酶。

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