Metabolically engineered and genetically transformed microorganisms, and method for producing a desired metabolic product

摘要

"Metabolically Engineered and Genetically Transformed Microorganisms, and Method to Produce a Desired Metabolic Product" A metabolically engineered microorganism has a first operative metabolic pathway in which a first metabolite is transformed into a second metabolite into a reaction in which NAD is a cofactor for a first enzyme, suitably a phosphorylation dehydrogenase, said reaction step producing NADH. Said second metabolite is transformed into at least one other metabolite into a reaction catalyzed by a second enzyme, suitably a kinase. The organism has a second operative metabolic pathway characterized by an enzyme activity in excess of a native level relative to a third enzyme, suitably a non-phosphorylation dehydrogenase, for example GAPN, which catalyzes a non-reversible reaction in which NADP is a cofactor. and NADPH is a product. Said first metabolite is transformed into said other metabolite without the involvement of said second enzyme.