Process for the preparation of a DNA fragment that encodes a polypeptide of human brain activity and possesses natriuretic polypeptide; Preparation procedure; DNA fragment; POlipeptido.

摘要

A process for the preparation of a DNA fragment that encodes a polypeptide derived from Human Brain and possesses natriuretic activity and have the following sequence of AminoacidYou.Be pro Lys GLN Gly met Val Gly Gly Lys ARG be ccys PHE met ARG ASP Gly Leu will be be be be Leuy CYS Gly Lys ARG ARG Val his,Procedure that involves the following stages (Isolate) to construct a cDNA bank by means of the following Operations: (1) to isolate mRNA from Human Brain or human Atrium through dAnd homogenization.Isolation by ultracentrifugation and purification (2) sintetizaz cDNA from mRNA obtained (3) apply an endonuclease ecori Adapter of the cDNA obtained after foThe sforolar End (4) Link using a Vector with which the Bank is built to trace the PNC Human Clone using a cDNA fragment as a Probe to hybridize Pig marking or both.Ra (c) selecting the positive Clone from a positive Clone selected with a suitable endonuclease to obtain the desired DNA fragment.