Recombinant or synthetic DNA sequence encoding an FGF protein, vector containing the DNA sequence and a recombinant host cell containing the DNA sequence or vector

摘要

Isolated, cloned recombinant or synthetic DNA sequence encoding acidic or basic mammalian fibroblast growth factors (FGF) are claimed. A DNA sequence, defined above, and including a DNA encoding a heterologous signal sequence upstream of, and operably linked to, the DNA encoding the FGF is also new. It may be operably linked to control sequences for expression. Recombinant host cells transformed with the DNA sequence are claimed. Recombinant vector contg., and effective in expressing, isolated etc. DNA sequence, the DNA sequence encoding FGF being operably linked to control sequences compatible with bacteria or mammalina hosts, is claimed. Prodn. of acidic or basic mammalian FGF comprises cultivation of transformed cells is claimed. Human basic FGF inpurified or recombinant form is claimed.