PROCESS FOR PRODUCING LEAF FERTILIZER COMPOSITIONS BASED ON LIGNIN SULPHONATE PREFERABLY POLLUTANT, WASTE LIGNIN SULPHONATE, AND THE PRODUCT MADE BY SAID PROCESS

摘要

Washed and chopped-up horse-radish or horse-radish skin is immersed in an aqueous salt solution containing 20-500 mg Ca2+ ions and 0.3-0.8 % by mass of non-ionic material with active surface to dissolve the soluble ingredients. The solution is separated and mixed with an aqueous solution of 0.5-1.5 parts by mass of sodium acetate. Chilled ethyl alcohol, 96 % concentrated, is added to the mixture, 100-200 parts by mass. At this point the mixture is kept at 0 [deg]C and the precipitate is separated. The filtrate is mixed with chilled ethyl alcohol, 96% concentrated, and the precipitate (which is raw horse-radish peroxidase enzyme) is again separated. The precipitate is dissolved in an aqueous solution, 30-100 parts by mass, containing 0.01-0.1 parts by mass of sodium acetate and 0.001-0.002 parts by mass of calcium chloride. The solution is purified in an ion-exchange column. Finally, horse-radish peroxidase enzyme is precipitated from the purified solution by mixing with 96 % concentrated, chilled ethyl alcohol, 1.5-4 parts by mass. The product is separated and dried.