SYNERGISTIC COMPOSITION FOR REDUCING SPERM MOTILITY AND A METHOD THEREOF

摘要

The present invention relates to a synergistic composition useful for reducing motility of mammalian spermatozoa and reversing the same, without having any deleterious effect on the mammalian spermatozoa and using the said composition for an easy pick up of mammalian spermatozoa in the intracytoplasmic sperm injection (ICSI) and for sperm banks, said composition comprising (a) sodium hydrogen phosphate buffer having disodium hydrogen phosphate (Na2HPOa) ranging between 0.23 - 0.27 gms and sodium dihydrogen phosphate (NaH2P04.H20) ranging between 0.023-0.027 gms, (b) sodium chloride ranging between 0.8 to 1.0 gms, and (c) 30% hydrogen peroxide ranging between 6.5 - 8.0 ml, and adding water to make up total volume to 100ml,with pH of said composition ranging between 7.0 to 7.4 and, a method of producing the composition thereof.