Identifying genetically modified mammalian cells with a cell surface mutated muscle specific tyrosine kinase receptor (MuSK-R) operatively linked to a promoter

摘要

Described is a method of using a muscle specific tyrosine kinase receptor molecule (MuSK-R) or a mutated MuSK-R (mMuSK-R) thereof as a selectable marker in mammalian cells, particularly human cells. Preferred markers are mMuSK-Rs incapable of signal transduction and preferably molecules wherein the intracellular domain has been modified by deletion of the signalling region. A method of identifying genetically modified mammalian cells including introducing a mMuSK-R into a target cell as a selectable marker is also described. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising identifying the transduced cells by incubation of the cells with an antibody which recognises and binds specifically to a MuSK-R or mMuSK-R thereof.